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Appl Environ Microbiol. 1968 August; 16(8): 1215-1219
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Identification of Stimulants for Lactobacillus bulgaricus in Tomato Juice1

T. M. Cogan, S. E. Gilliland and M. L. Speck

Department of Food Science, North Carolina State University, Raleigh, North Carolina 27607

ABSTRACT

Acid production in milk by Lactobacillus bulgaricus was stimulated by tomato juice or its serum. Preliminary purification of the stimulants involved adsorption and elution on a cation-exchange resin. Unidimensional paper chromatography in two solvent systems was employed in further isolation and purification. Identification of the stimulatory components was based on ultraviolet spectral analysis and thin-layer chromatography. The stimulants were identified as adenine and adenosine.

FOOTNOTES

1 Paper no. 2609 of the Journal Series of the North Carolina State University Agricultural Experiment Station, Raleigh.

Appl Environ Microbiol. 1968 August; 16(8): 1215-1219
Copyright © 1968 American Society for Microbiology. All Rights Reserved.





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Copyright © 1968 by the American Society for Microbiology. All rights reserved.