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Appl Environ Microbiol. 1968 August; 16(8): 1211-1214
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Detoxification of Liquid Cultures of Bordetella pertussis by Forced Aeration at High pH

Commonwealth Serum Laboratories, Parkville, Victoria, Australia

ABSTRACT

Cultures of Bordetella pertussis cultivated in shake flasks were invariably highly toxic for mice, but cultures of the same strain grown in vortex-aerated vessels were nontoxic at the time of harvest. Results reported here indicate that toxin is present during the early log phase in vortex-aerated cultures, but is lost as the cultivation proceeds. The loss of toxicity is apparently due to denaturation of the toxin by the combined influence of vigorous aeration and elevated pH.