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Appl Environ Microbiol. 1968 August; 16(8): 1163-1167
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Bioproduction and Purification of Rubratoxin

Division of Toxicology, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37203

ABSTRACT

Methods were developed for bioproduction and extraction of rubratoxin B from liquid cultures of Penicillium rubrum P-13 (NRRL A-11785). A maximum of 874.7 mg of toxin per liter of medium was attained in 21 days using stationary cultures of Mosseray's simplified Raulin solution enriched with 2.5% malt extract. Malt extract was required for rubratoxin production. Rubratoxin was not produced in either shake flasks or in fermentors with restricted aeration. Crystalline toxin was obtained by liquid-liquid extraction of concentrated culture medium with ethyl ether. Adhering colored impurities were removed by column chromatography and by recrystallization from acetone.