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Appl Environ Microbiol. 1968 July; 16(7): 981-987
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Production of Nucleic Acid-related Substances by Fermentative Processes

XIX. Accumulation of 5'-Inosinic Acid by a Mutant of Brevibacterium ammoniagenes

Tokyo Research Laboratories, Kyowa Fermentation Industry Co., Ltd., Machida-shi, Tokyo, Japan

ABSTRACT

The accumulation of 5'-inosinic acid (IMP) by a mutant, KY 13102, induced from Brevibacterium ammoniagenes ATCC 6872 by ultraviolet light irradiation, was examined. Although growth was stimulated by adenine or adenosine, the microorganism showed fair growth in the medium containing amino acids but no adenine. Among six kinds of natural nutrients tested, meat extract and Casamino Acids were suitable for the accumulation of IMP. Manganese ion strongly affected growth, the accumulation of IMP and hypoxanthine, and cell morphology. Among amino acids tested, L-methionine, L-proline, and L-valine stimulated IMP accumulation. In the medium containing 1.0 g of L-proline per liter, 12.8 mg of IMP per ml was accumulated. The mechanism of IMP accumulation by the mutant is discussed.