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Appl Environ Microbiol. 1968 July; 16(7): 1053-1055
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Assay for Aflatoxin Production by the Genera Aspergillus and Penicillium¹

Department of Botany and Plant Pathology, Purdue University, Lafayette, Indiana 47907

ABSTRACT

A total of 260 isolates, including 43 species of Penicillium and 7 species of Aspergillus, were screened for their ability to produce aflatoxin on rice. Chloroform extracts were analyzed by thin-layer chromatography. None of the isolates produced aflatoxin. Certain species of Penicillium produced fluorescent substances that either were similar in R_F or were of similar color to B and G aflatoxins. These substances were subsequently proved not to be aflatoxin by two-dimensional chromatography, by reaction with iodine fumes, or by both methods.

¹ Journal paper no. 3350, Purdue University Agricultural Experiment Station.