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Appl Environ Microbiol. 1968 July; 16(7): 1036-1039
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Microtiter Method for the Evaluation of Viable Cells in Bacterial Cultures¹

Department of Dairy and Food Industry, Iowa State University, Ames, Iowa 50010

ABSTRACT

A microtiter technique was investigated as a means of evaluating viable cells in bacterial cultures. Parallel experiments were performed employing the conventional agar plate method along with the microtiter procedure. Statistical analyses showed that the correlation between the two methods was highly significant. With this new method, many samples were analyzed simultaneously, and readable results were obtained in 12 to 15 hr. Other advantages of this method were substantial savings of time, space, and materials. Also, the applicability of this method to estimates of mixed bacterial populations was demonstrated by studying the associative growth of two bacterial cultures.

¹ Journal Paper No. J-5899 of the Iowa Agriculture and Home Economics Experiment Station, Ames. Project No. 1392; Center for Agricultural and Economic Development Cooperating.