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Appl Environ Microbiol. 1968 June; 16(6): 912-916
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Liquid Nitrogen Preservation of Standard Inoculum: Gas-Phase Storage

Institute of Microbiology, Rutgers, The State University, New Brunswick, New Jersey 08903

ABSTRACT

Liquid nitrogen storage was the most satisfactory of several methods tested for supplying standard Streptomyces viridoflavus inoculum for laboratory and pilot plant experimentation. Shake-flask cultures were subdivided into sterile cotton-plugged ampoules and stored in the gas space of a liquid nitrogen refrigerator. There were no detectable changes in viability or in candidin-producing capacity over a 12-month test period. The procedure also proved satisfactory with all other organisms tested.