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Appl Environ Microbiol. 1968 June; 16(6): 890-895
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Effect of Different Media on In Vitro Studies of Antibiotic Combinations

Roger J. Bulger and Kathleen Nielson

Department of Medicine, University of Washington, Seattle, Washington 98105

ABSTRACT

In an effort to determine the adequacy of a standard broth medium in the evaluation of antibiotic combinations, 20 strains of various bacterial species were studied simultaneously in Mueller-Hinton broth and in freshly drawn human serum from apparently healthy volunteers. Studies of growth dynamics by use of the usual plate dilution technique for quantitating colony-forming units were performed with strains of Staphylococcus aureus (methicillin-resistant and methicillin-susceptible), Streptococcus faecalis, Escherichia coli, Aerobacter, Klebsiella, and Proteus mirabilis. A variety of different antibiotics were investigated. With 19 of the 20 strains, interpretations of synergism or antagonism were the same in both media. Therefore, despite minor variations when the same strain was studied in both serum and broth, it is concluded that Mueller-Hinton broth is an adequate medium for use in studies of chemotherapeutic combinations in vitro. A simplified method for studying bactericidal activity is described, which is deemed practical for clinical microbiology laboratories and which led to the same conclusions regarding the combinations as were obtained by the more arduous plate dilution test.

Appl Environ Microbiol. 1968 June; 16(6): 890-895
Copyright © 1968 American Society for Microbiology. All Rights Reserved.





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