This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hunt, D. E. Articles by Pittillo, R. F. Search for Related Content PubMed Articles by Hunt, D. E. Articles by Pittillo, R. F. Agricola Articles by Hunt, D. E. Articles by Pittillo, R. F.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1968 June; 16(6): 867-870
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

A Microbiological Assay for Estimating Concentrations of Camptothecin Lactone in Mouse Tissues

Kettering-Meyer Laboratories,¹ Southern Research Institute, Birmingham, Alabama 35205

ABSTRACT

The marked sensitivity of the yeast Hansenula wingei NRRL Y-2340 strain 5 to a new antineoplastic alkaloid served as the basis for a logarithmic-ratio microbiological assay which has been developed for the estimation of concentrations of camptothecin lactone (or cytotoxic equivalents) in tissues of mice. The utility of this assay has been demonstrated by presenting data which show the concentrations of camptothecin lactone (or cytotoxic equivalents) detected in various tissues of mice at intervals following intraperitoneal injection of a 1 or 0.5 LD₁₀ dose of this drug.

¹ Affiliated with the Sloan-Kettering Institute for Cancer Research.