This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Yoshida, F. Articles by Ichishima, E. Search for Related Content PubMed Articles by Yoshida, F. Articles by Ichishima, E. Agricola Articles by Yoshida, F. Articles by Ichishima, E.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1968 June; 16(6): 845-847
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Effect of Lipid Materials on the Production of Lipase by Torulopsis ernobii

Laboratory of Enzymology, Central Research Institute, Kikkoman Shoyu Co., Ltd. Noda, Chiba-prefecture, Japan

ABSTRACT

The yeast Torulopsis ernobii produced approximately 50 units of extracellular lipase (glycerol ester hydrolase, E.C. 3.1.1.3) per ml when grown in 30-liter fermentors at 33 C for 40 hr in a base medium at pH 5.0. The addition of fats, oils, triglycerides, or higher fatty acids to this medium at concentrations of 0.2 to 0.6% markedly increased production; twofold increases in yield were obtained when 0.2% olive oil or a mixture of 0.14% oleic acid and 0.04% palmitic acid was added. The production of lipase paralleled growth, and the role of lipid materials in augmenting lipase production appears to be related to cell growth.