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Appl Environ Microbiol. 1968 June; 16(6): 841-844
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

A Precise Method for Replicating Suspension Cultures of Mammalian Cells¹

Department of the Army, Fort Detrick, Frederick, Maryland 21701

ABSTRACT

A simple, readily assembled shaker-culture system for the cultivation of mammalian cells is described. No specialized glassware and equipment were used in this system, which consists of an Erlenmeyer flask fitted with a breather-sampling assembly. This unit was employed to quantitate the effects of several variables, including medium ingredients, serial transfers, and freezing and storage on two variants of the L-cell line. This system is reproducible and precise and allows for growth of cells in suspension for extended periods of time. Large numbers of cells can be mass-produced. Many replicates can be run simultaneously to yield data for statistical analysis.

¹ Presented in part at the 67th Annual Meeting of the American Society for Microbiology, New York, N.Y., 1 May 1967.