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Appl Environ Microbiol. 1968 May; 16(5): 703-707
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Hemagglutinating Activity of Enteroviruses Recovered in Two Primary Cell Lines and a Stable Cell Line

Phoenix Field Station Section, Ecological Investigations Program, National Communicable Disease Center, Phoenix, Arizona 85014

ABSTRACT

A microhemagglutination technique was used to detect hemagglutinating properties of enteroviruses recovered in two primary cell lines, monkey kidney (MK) and human amnion (HAm), and in a continuous cell line, human embryonic lung (HEL). During a 3-year period, 1,528 isolations of enteroviruses were tested for hemagglutinating activity and hemagglutination inhibition response; 96.3% of the viruses were also identified by virus neutralization tests. Enteroviruses recovered in HEL were far less likely to develop hemagglutinins than viruses isolated in MK or HAm. Of the enteroviruses known to agglutinate human type O cells, 77.8% of the primary viral isolates from MK, 62.1% of the isolates from HAm, and 20.3% of the isolates from HEL exhibited this property. An additional 8.1% of the isolations obtained in HEL hemagglutinated human cells after a single passage in MK. The microhemagglutination technique using microtiter equipment was simple to perform, saved time and valuable typing sera, and helped to obtain identifications rapidly.

¹ Present address: Clinical Microbiology, University Hospital, University of Washington, Seattle 98105.