This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Albizo, J. M. Articles by Surgalla, M. J. Search for Related Content PubMed Articles by Albizo, J. M. Articles by Surgalla, M. J. Agricola Articles by Albizo, J. M. Articles by Surgalla, M. J.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1968 April; 16(4): 649-655
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Specific Identification of Fraction I-Positive Pasteurella pestis Colonies on Antiserum-Agar Plates

Biological Sciences Laboratory, Fort Detrick, Frederick, Maryland 21701

ABSTRACT

A method is described for the use of antiplague serum in Blood Agar Base plating media to detect fraction I-positive Pasteurella pestis. The antiserum was produced conveniently and in large volume in rabbits by use of Cutter plague vaccine combined with Freund's complete adjuvant. P. pestis colonies were specifically identified within 48 hr after plating by the presence of a precipitin ring surrounding each colony. The basis of the test was shown to be a precipitin reaction between fraction I antigen released from P. pestis colonies after chloroform vapor treatment and fraction I antibody present in the antiserum-agar medium.