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Appl Environ Microbiol. 1968 April; 16(4): 577-578
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

A New Plating Medium for the Isolation of Enteric Pathogens

I. Hektoen Enteric Agar

Department of Microbiology, Cook County Hospital, Chicago, Illinois 60612
The Hektoen Institute for Medical Research, Chicago, Illinois 60612

ABSTRACT

A new agar plating medium for the isolation of enteric pathogens is described. This medium contains greater quantities of peptone to offset the inhibitory effects of bile salts. Additional carbohydrates and larger quantities of those previously used have also been incorporated into the medium to differentiate pathogens from some of the slow lactose fermenters. The use of an indicator system never used before in a differential plating medium opens new possibilities for improvement over traditional and newer selective media. Known strains from the various genera of Enterobacteriaceae have been tested for colonial differentiation as well as for possible inhibitory effects. The results show the medium to be highly selective for the shigellae as well as for other enteric pathogens.