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Appl Environ Microbiol. 1968 February; 16(2): 326-329
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Production of Alkaline Phosphatase from Escherichia coli in Continuous Culture

P. D. Sayer1

Microbiological Research Establishment, Porton, Wiltshire, United Kingdom

ABSTRACT

A 2-liter continuous culture for the production of Escherichia coli rich in alkaline phosphatase is described. The maximal output is greater than 250 mg of enzyme per day. The enzyme yield per unit of bacterial dry weight is only one-fifth of that obtained in earlier investigations. However, because of the increased bacterial density, the output per unit volume of culture is more than four times as great.

FOOTNOTES

1 Present address: Dista Products Ltd., Speke, Liverpool 24, England.

Appl Environ Microbiol. 1968 February; 16(2): 326-329
Copyright © 1968 American Society for Microbiology. All Rights Reserved.





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Copyright © 1968 by the American Society for Microbiology. All rights reserved.