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Appl Environ Microbiol. 1968 February; 16(2): 296-300
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Production of Clostridium pasteurianum in a Defined Medium

Microbiological Research Establishment, Porton, Wiltshire, United Kingdom

ABSTRACT

A method for the growth of Clostridium pasteurianum in a 140-liter (total capacity) stainless-steel vessel is described. By preventing the pH value from falling below 5.6, the growth of cultures was prolonged. Larger amounts of the carbon source (sucrose) and the nitrogen source (ammonium ion) were supplied and consumed, and cell yields of up to 5.56 g (dry weight) per liter were obtained. The highest cell yield previously reported was 1.7 g (dry weight) per liter obtained under nitrogen-fixing conditions in 500-ml cultures. The ferredoxin content of the cells was comparable with that obtained by earlier workers.