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Appl Environ Microbiol. 1968 February; 16(2): 285-290
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Direct Quantitative Gas Chromatographic Separation of C₂-C₆ Fatty Acids, Methanol, and Ethyl Alcohol in Aqueous Microbial Fermentation Media

Laboratory of Microbiology, National Institute of Dental Research, Bethesda, Maryland 20014

ABSTRACT

A method is described for the direct quantitative gas chromatographic separation of C₂-C₆ lower fatty acid homologues, methanol, and ethyl alcohol in aqueous microbial fermentation media. A hydrogen flame detector and a single-phase solid column packing, comprising beads of a polyaromatic resin (polystyrene cross-linked with divinyl benzene), were employed. Direct injections of 1 to 10 µliters of aqueous culture supernatant fluids were made. Quantitative recoveries of C₂-C₆ acids added to culture supernatant fluids were obtained.