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Appl Environ Microbiol. 1968 February; 16(2): 279-284
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Rapid Method for Characterization of Actinomycetes by Cell Wall Composition

National Communicable Disease Center, Atlanta, Georgia 30333

ABSTRACT

To achieve a rapid identification of the cell wall components of actinomycetes, several modifications of the procedure of Cummins and Harris were developed. Purified cell walls were prepared by extraction of whole cells with 0.1 N NaOH. A 2-hr 2 N HCl hydrolysate was prepared for identification of sugars. A 2-hr 6 N HCl hydrolysate was prepared for amino acid analysis. Two-dimensional paper chromatography was run on 19-cm papers. Total time required for a single analysis was approximately 24 hr. This procedure gave qualitative results which were completely satisfactory for differentiation of certain species in the genus Actinomyces and in related genera.