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Appl Environ Microbiol. 1968 November; 16(11): 1734-1737
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Detection and Incidence of Specific Species of Spoilage Bacteria on Fish

I. Methodology

Department of Food Science and Technology, University of Massachusetts, Amherst, Massachusetts 01002

ABSTRACT

The ability of Pseudomonas putrefaciens to form H₂S was found to serve as a singularly useful criterion of identity for this species and was used to directly enumerate the organism from haddock fillets by the use of pour plates of Peptone-Iron Agar. Subsurface colonies appear intensely black, whereas surface colonies are black or gray. A highly sensitive soft-agar-gelatin overlay technique has been found useful for directly determining the numbers of weakly and strongly proteolytic organisms from fish tissue.