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Appl Environ Microbiol. 1968 November; 16(11): 1713-1718
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Quantitative Analysis of Actinomyces Cell Walls

Martha S. DeWeese, Mary Ann Gerencser and John M. Slack

Department of Microbiology, Medical Center, West Virginia University, Morgantown, West Virginia 26506

ABSTRACT

Quantitative data on the amino acid composition of cell walls of five species of Actinomyces were obtained by using a Beckman-Spinco amino acid analyzer. The major amino acids in A. israelii, A. naeslundii, A. eriksonii, and A. bovis species included alanine, glutamic acid, lysine, aspartic acid, and ornithine, as reported by previous workers, whereas A. propionicus contained diaminopimelic acid. Other amino acids, including glycine, valine, leucine, proline, isoleucine, and threonine, were present in at least some of the walls in quantities equal to or slightly less than that of lysine. This raised the question of whether these may represent cross-links in the peptidoglycan or other cell wall structural components or whether the wall preparations contained nonpeptidoglycan material despite the use of electron microscopy as a standard of purity; further work is required to supply the answer. The quantitative data furnish relative molar concentrations of amino acids, which can provide definitive identification of some of the species and differentiation of Actinomyces from other members of the Actinomycetales and from morphologically similar genera such as Corynebacterium and Propionibacterium.

Appl Environ Microbiol. 1968 November; 16(11): 1713-1718
Copyright © 1968 American Society for Microbiology. All Rights Reserved.





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