This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hebeka, E. K. Articles by Beardmore, W. B. Search for Related Content PubMed Articles by Hebeka, E. K. Articles by Beardmore, W. B. Agricola Articles by Hebeka, E. K. Articles by Beardmore, W. B.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1968 November; 16(11): 1699-1705
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Automated Procedure for Measuring Antigenicity of Extracted and Intact Influenza Virus

Research Laboratories, Parke, Davis & Company, Detroit, Michigan 48232

ABSTRACT

An automated serum-blocking (S-B) technique was developed in an attempt to find an in vitro test for the determination of the antigenicity of extracted influenza vaccines. The S-B test depends on the ability of an antigen to combine with (or block) specific (in this case, hemagglutination-inhibiting) antibodies. After mixing the test virus with a constant amount of specific antiserum and hemagglutinating virus in an Auto Analyzer, chicken erythrocytes were pumped into the system and the mixture was incubated by passing through coils. The hemagglutinated cells were removed and the residual cells were lysed. The optical density was read and recorded automatically. The S-B test was much more reproducible than the chicken cell agglutination (CCA) test. There was good correlation between the S-B and CCA titers of intact influenza virus, but not of ether-extracted influenza virus. The CCA titer of influenza strains of type A was reduced significantly during ether-extraction. The S-B titers indicated that there was no significant loss in specific antigenicity when influenza strains of types A and B were extracted with ether and Tween-80 according to the described procedure. The S-B test seemed to be a true measurement of the total antigens present in influenza vaccines.