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Appl Environ Microbiol. 1968 November; 16(11): 1650-1654
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Air Filters for Germ-free Isolators

Department of Surgery, University of Wisconsin Medical School, Madison, Wisconsin 53706

ABSTRACT

A germ-free isolator must have a perfect bacterial filter. This paper describes a new, relatively inexpensive, stainless-steel filter frame, which is easily and quickly assembled and protects the enclosed filter material at all times. Resistance to the flow of air was less than 4 inches of water at an airflow of 30 ft³/min through the filter frame with 204 inches² of surface area and four, one-half inch thick pieces of fiberglass filter material. This filter performed satisfactorily in our gnotobiotic laboratory and was found to be consistently 100% efficient in removing an aerosol containing Serratia marcescens from an air stream under a variety of operating conditions.