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Appl Environ Microbiol. 1968 October; 16(10): 1584-1590
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Purine Analogue Sensitivity and Lipase Activity of Leptospires

Department of Microbiology, University of Minnesota, Minneapolis, Minnesota, 55455

ABSTRACT

The genus Leptospira can be divided into three groups based on purine analogue sensitivity and lipase (trioleinase) activity. Group 1 contains members of the "parasitic complex" of leptospires which initially cannot grow in media containing 10 µg of 2,6-diaminopurine (DAP) per ml or 200 µg of 8-azaguanine per ml. In addition, leptospires in this group possess lipase activity. Group 2 also contains members of the "parasitic complex" of leptospires. Although these leptospires are similarly sensitive to 8-azaguanine, they differ from group 1 leptospires in that they grow in media containing 10 µg of DAP per ml, and they do not possess detectable lipase activity. Group 3 consists of leptospires belonging to the "biflexa complex." These leptospires are resistant to both purine analogues and have lipase activity.