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Appl Environ Microbiol. 1968 October; 16(10): 1575-1583
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Production of Reference Enteroviruses

Division of Microbiology and Infectious Diseases, Southwest Foundation for Research and Education, San Antonio, Texas 78206

ABSTRACT

Forty-five human enterovirus reagents of certified purity and quality were prepared for use as seed viruses and as immunizing antigens. One of the reagents was ampouled as "untreated" seed virus, whereas 14 were ampouled as "MgCl₂-stabilized" reagents. The remaining 30 reagents were ampouled as "untreated" seed viruses and as "MgCl₂-stabilized" reagents. Thirty of the reagents were propagated on primary African green monkey kidney cells, 3 on primary baboon kidney cells, 3 on primary rhesus monkey kidney cells, and the remaining 9 on human amnion cells. Forty-two of the viral antigens were concentrated for use in the production of high-titered specific antisera in large animals.