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Appl Environ Microbiol. 1968 October; 16(10): 1528-1531
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

New Diagnostic System for the Identification of Lactose-fermenting Gram-negative Rods¹

Department of Microbiology, Isaac Albert Research Institute, Kingsbrook Jewish Medical Center, Brooklyn, New York 11203

ABSTRACT

The identification of prompt lactose-fermenting gram-negative rods has generally relied heavily upon colonial morphology coupled with one or more indole, methyl red, Voges-Proskauer, citrate (IMViC) parameters, hydrogen sulfide, and motility. Studies were undertaken to compare diagnoses dependent solely upon the more orthodox criteria to a system for identification based upon hydrogen sulfide, ornithine decarboxylase, and citrate utilization (HOC). The results suggest that the IMViC scheme of identification is neither consistent nor applicable when applied to the current nomenclature of the above group of organisms and should be discarded, whereas the HOC system may prove to be of significant value to clinical microbiologists.

¹ Presented in part at the 68th Annual Meeting of the American Society for Microbiology, Detroit, Mich., May 1968.