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Appl Environ Microbiol. 1968 October; 16(10): 1475-1477
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Simple Method to Remove Completely Ciliate Protozoa of Adult Ruminants¹

Division of Biology and Department of Dairy and Poultry Science, Kansas State University, Kansas

ABSTRACT

Dioctyl sodium sulfosuccinate (Aerosol OT) has been used in a remarkably short period to obtain rumen ciliate-free cows. Two 30-g doses of Aerosol OT given on consecutive days appeared to effectively eliminate all types of rumen ciliate protozoa without harming the health of the host animal. Measurement of the rumen metabolic parameters of gas production, carbon dioxide to methane ratio, pH value, volatile fatty acids, ammonia, and in vitro cellulose digestion, along with total amylolytic streptococci counts of the rumen contents, showed that conditions in rumens of defaunated animals are normal 4 days after such treatment. It appears that such animals may be used in ruminal studies that require defaunated animals.

² Permanent address: Department of Animal Production, Faculty of Agriculture, University of Alexandria, Alexandria, Egypt.

¹ Contribution 971, Division of Biology, and 689, Department of Dairy and Poultry Science, Kansas Agricultural Experiment Station, Manhattan, Kansas.