This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Harada, T. Articles by Misaki, A. Search for Related Content PubMed Articles by Harada, T. Articles by Misaki, A. Agricola Articles by Harada, T. Articles by Misaki, A.

Next Article 

Appl Environ Microbiol. 1968 October; 16(10): 1439-1444
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Formation of Isoamylase by Pseudomonas

The Institute of Scientific and Industrial Research, Osaka University, Suita-shi, Osaka, Japan

ABSTRACT

We have isolated a Pseudomonas sp. (strain SB15) which produces an isoamylase (EC 3.2.1.9). Highest yields of this enzyme were obtained when the bacterium was grown in shaken culture in a medium containing maltose, dextrin, starch, or isomaltose. Specific carbon and nitrogen sources were required for growth. The most satisfactory medium consisted of 2% maltose, 0.4% sodium glutamate, 0.3% diammonium hydrogen phosphate, and other inorganic salts. The optimal pH for enzyme production was 5 to 6. The enzyme is stable between pH 3 and 6 but is extremely labile above pH 7. It splits amylopectin completely by combined action with ß-amylase but does not attack pullulan.