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Appl Environ Microbiol. 1968 January; 16(1): 53-55
Copyright © 1968 American Society for Microbiology. All Rights Reserved.
Biological Sciences Laboratory, Department of the Army, Fort Detrick, Frederick, Maryland 21701
ABSTRACT
Procedures are described for preparation of a chemically defined medium, capable of being autoclaved, for the cultivation of animal cells in suspension. Yields of 34.1 x 105, 33.8 x 105, and 47.1 x 105 cells per ml were recorded for cat kidney, HeLa, and mouse fibroblast (L) cells after 6, 10, and 10 days of incubation, respectively.
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