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Appl Environ Microbiol. 1968 January; 16(1): 53-55
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Heat-stable Chemically Defined Medium for Growth of Animal Cells in Suspension

Stanley C. Nagle Jr.

Biological Sciences Laboratory, Department of the Army, Fort Detrick, Frederick, Maryland 21701

ABSTRACT

Procedures are described for preparation of a chemically defined medium, capable of being autoclaved, for the cultivation of animal cells in suspension. Yields of 34.1 x 105, 33.8 x 105, and 47.1 x 105 cells per ml were recorded for cat kidney, HeLa, and mouse fibroblast (L) cells after 6, 10, and 10 days of incubation, respectively.


Appl Environ Microbiol. 1968 January; 16(1): 53-55
Copyright © 1968 American Society for Microbiology. All Rights Reserved.







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Copyright © 1968 by the American Society for Microbiology. All rights reserved.