This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Rehácek, Z. Articles by Kozová, J. Search for Related Content PubMed Articles by Rehácek, Z. Articles by Kozová, J. Agricola Articles by Rehácek, Z. Articles by Kozová, J.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1968 January; 16(1): 29-32
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Respiratory Chain of Antimycin A-producing Streptomyces antibioticus

Z. eháek, M. Ramankutty¹ and Jaroslava Kozová

Department of Biogenesis of Natural Products, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague, Czechoslovakia

ABSTRACT

An active respiratory chain system was demonstrated in sonically treated mycelium of Streptomyces antibioticus, the producer of antimycin A. The respiratory electron transfer from substrate to oxygen proceeded successively through flavoprotein(s), b-, c-, and a-type cytochromes, and terminated with the cyanide-sensitive cytochrome oxidase. The cytochrome composition of the culture was not affected by the age of the mycelium, the intensity of antimycin A production, or differences in the media. Slater factor, coenzyme Q, and vitamin K were not interposed as hydrogen carriers in the respiratory chain between flavoproteins and cytochromes. The oxidation of reduced nicotinamide adenine dinucleotide and succinate was unaffected by antimycin A. Evidence is presented in support of the absence of the antimycin A-sensitive site from the electron transport system of S. antibioticus.

¹ Present address: Antibiotics Division, Central Drug Research Institute, Lucknow, India.