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Appl Environ Microbiol. 1968 January; 16(1): 21-24
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Detection of Botulinal Toxins by Immunodiffusion¹

Department of Dairy and Food Industry, Iowa State University, Ames, Iowa 50010

ABSTRACT

A procedure employing concentration with Sephadex and analysis by gel diffusion (Ouchterlony) was used to detect the toxins of Clostridium botulinum in foods. Botulinal toxins with toxic levels of 370 to 557 mouse LD₅₀ per milliliter were detected in the food samples. Test results were verified by use of the mouse protection test. Approximately 24 hr were required to complete the entire procedure.

¹ Journal Paper No. J-5707 of the Iowa Agricultural and Home Economics Experiment Station, Ames, Iowa, in cooperation with Project No. 1393, Center for Economic and Agricultural Development.