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Appl Environ Microbiol. 1963 January; 11(1): 33-35

Continuous-Flow Mechanical Cell Disintegrator

John W. Ross

The Squibb Institute for Medical Research, New Brunswick, New Jersey

ABSTRACT

A mechanical cell disintegrator is described which efficiently ruptures bacteria, molds, actinomycetes, and yeasts. Cells were broken continuously in a chamber by rapid shaking of a mixture of cell suspension, plastic beads, and air. Volume capacity for complete breakage varied with experimental conditions and the microorganism involved, and ranged from 840 ml/hr to greater than 4,470 ml/hr. Subcellular preparations made with the machine retained a high level of enzymatic activity.

Appl Environ Microbiol. 1963 January; 11(1): 33-35






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