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Appl Environ Microbiol. 1962 November; 10(6): 580-582

Continuous Culture of Ruminal Microorganisms in Chemically Defined Medium¹

I. Design of Continuous-Culture Apparatus

Bacteriology Department, Iowa State University, Ames, Iowa

ABSTRACT

An apparatus is described which has been used for successful continuous culture of the ciliates from the rumen of cattle. Automatic control of feeding rate, pH, oxidation-reduction potential, temperature, stirring rate, aeration rate, salinity, and volume of the culture is provided for, using standard commercial equipment, whenever possible. The operation of this apparatus is described.

¹ Journal Paper No. J-4390 of the Iowa, Agricultural and Home Economics Experiment Station, Ames, Iowa. Project 1208. Supported in part by funds provided by Regional Project NC-25.