This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hamilton, P. B. Articles by Knight, S. G. Search for Related Content PubMed Articles by Hamilton, P. B. Articles by Knight, S. G. Agricola Articles by Hamilton, P. B. Articles by Knight, S. G.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1962 November; 10(6): 577-579

Ballistic Disruption of Penicillium chrysogenum Cells

Department of Bacteriology, University of Wisconsin, Madison, Wisconsin

ABSTRACT

The use of glass beads in a high-speed mixing device to rupture organisms was applied to molds. The use of a mixer in which the propellor shaft enters from the top into a metal mixing chamber made it possible to immerse the whole device in a salt water and ice mixture so that the temperature of the glass-bead slurry could be kept below 5 C without difficulty. Mycelia, glass beads, and buffer in a 1:2:3 (w/w) ratio gave above 95% breakage in 15 min with Penicillium chrysogenum cells and in 4 min with Rhizopus nigricans. Some of the factors influencing breakage are discussed.

¹ Present address: Sterling-Winthrop Research Institute. Rensselaer, N.Y.