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Appl Environ Microbiol. 1962 September; 10(5): 413-417

Statistical Measurement of Biotin, Thiamine, and Zinc Concentrations Required for Maximal Growth of Arthrobotrys conoides¹

Department of Agricultural Microbiology, Rutgers, The State University, New Brunswick, New Jersey

ABSTRACT

An experiment of central composite design was performed to determine simultaneously the most suitable concentration of each of three nutrients essential for proliferation of the nematode-trapping fungus, Arthrobotrys conoides, and to detect any significant interaction that occurred when they were supplied at different levels. Regression analyses of the results obtained revealed that for maximal growth of A. conoides in a glucose-inorganic salts medium, biotin, thiamine, and zinc were required at concentrations of 5, 100, and 400 µg/liter, respectively. No interaction of significance was detected. The analytical procedures are presented in detail, and the benefits that accrue from a statistical approach are discussed.

² Present address: Engineering Experiment Station, University of New Hampshire, Durham.

³ Present address: Bell Telephone Laboratories, Inc., Murray Hill, N.J.

¹ Paper of the Journal Series, New Jersey Agricultural Experiment Station, Rutgers, The State University, New Brunswick, N.J.