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Appl Environ Microbiol. 1962 July; 10(4): 281-288

Carbon Dioxide-Limited Growth of Chlorella in Continuous Culture¹

Northwestern Technological Institute, Evanston, Illinois

ABSTRACT

The original mathematical model of microbial growth in the Chemostat is modified to describe continuous cultures in which the limiting nutrient is supplied at a rate independent of the dilution rate. The application of this model to continuous Chlorella cultures in which CO₂ is the factor limiting growth is discussed. The conclusions from the model are: (i) at a constant rate of CO₂ supply, the equilibrium population density is directly proportional to the retention time; and (ii) the production rate is directly proportional to the rate of CO₂ supply. Experimental data are presented to verify the theoretical conclusions.

¹ This paper was presented at the winter meeting of the Society of Illinois Bacteriologists, Evanston, Ill., Feb. 17, 1962.