This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Traxler, R. W. Articles by Shive, W. Search for Related Content PubMed Articles by Traxler, R. W. Articles by Shive, W. Agricola Articles by Traxler, R. W. Articles by Shive, W.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1962 March; 10(2): 99-101

Purification and Characterization of a Growth Stimulant for Bacillus Species¹

Department of Bacteriology and The Department of Chemistry, University of Texas, Austin, Texas

ABSTRACT

A procedure is described whereby the growth stimulatory material from an autoclaved solution of glucose and phosphate can routinely be purified 689-fold. Chemical characterization of this purified material indicates the presence of a hydroxyl group and possibly a carboxyl group on the active factor(s) which are associated with activity. Evidence is presented that there are two active factors or two forms of the same factor present in the concentrate.

² Present address: Department of Bacteriology, University of Southwestern Louisiana, Lafayette, La.

¹ This study was supported in part under a contract (AF-18(600)-933) with the U. S. Air Force School of Aviation Medicine, Randolph Field, Texas, and in part by The Institute For Biochemical Research, University of Texas.