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Appl Environ Microbiol. 1962 March; 10(2): 93-98

Production Model Press for the Preparation of Bacterial Cell Walls

National Institute of Arthritis and Metabolic Diseases and the National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland

ABSTRACT

A modification of the apparatus previously described permits the preparation of cell walls in quantity. This consists of a heavy duty, double-acting hydraulic press with motor-driven pump, and a superstrength alloy steel pressure cell which is corrosion resistant. Liquid cooling of the jet is substituted for the previously used gas cooling to minimize aerosol formation and to facilitate subsequent treatment of the products. The device produces cell walls of excellent quality in good yield. The pressure cell has been used satisfactorily up to about 60,000 psi. Design details are given.