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Appl Environ Microbiol. 1962 March; 10(2): 153-156

Production of a New Polysaccharide with Cryptoccus laurentii var. flavescens

Northern Regional Research Laboratory,¹ Peoria, Illinois

ABSTRACT

Conditions were investigated for the production of a new gum by the yeast Cryptococcus laurentii var. flavescens NRRL Y-1401 in shaken flasks and 20-liter fermentors. The most suitable medium contained 6% commercial glucose, 0.25% autolyzed brewer's yeast, and 0.001% MnSO₄ and had an initial pH of 6.5. Polysaccharide yields, as measured by the dry weight of the alcohol precipitates, were in the range of 30 to 35% based on initial glucose.

¹ This is a laboratory of the Northern Utilization Research and Development Division, Agricultural Research Service, U. S. Department of Agriculture.