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Appl Environ Microbiol. 1962 March; 10(2): 132-136

Microbiological Production of Carotenoids

VIII. Influence of Hydrocarbon on Carotenogenesis by Mated Cultures of Blakeslea trispora

Northern Regional Research Laboratory,¹ Peoria, Illinois

ABSTRACT

Synthesis of ß-carotene by mated strains of Blakeslea trispora in shaken-flask culture was considerably enhanced by adding either 5% kerosene after 2 days of fermentation or acid-refined kerosene at the start of fermentation to a grain-based medium that also contained a natural lipid, nonionic detergent, and ß-ionone; average yields of 17,500 µg per g of dry fermentation solids (86,000 µg per 100 ml of medium) were attained when refined kerosene was used. Almost all of the carotene was retained within the mycelium. Peak yields were achieved in 5 days.

¹ This is a laboratory of the Northern Utilization Research and Development Division, Agricultural Research Service, U. S. Department of Agriculture.