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Appl Environ Microbiol. 1962 January; 10(1): 60-64

Reproducibility of the Most Probable Numbers Technique for Determining the Sanitary Quality of Clams¹

Chesapeake Biological Laboratory, Solomons, Maryland

ABSTRACT

The reproducibility of most probable numbers results in the bacteriological examination of clam meats, using the tentatively recommended procedure, is less than has been indicated in use with water analysis. Entrapped air bubbles in ground clam meats contribute to the variability of results by (i) lowering the density of the sample material, producing inaccurate aliquots for inocula, and (ii) interfering with the attainment of homogeneity in the brei.

² Present address: Graduate School of Oceanography, Narragansett Marine Laboratory, University of Rhode Island, Kingston, R. I.

¹ This investigation was supported by U. S. Public Health Service research grant E-2228 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health. Contribution no. 194, Maryland Department of Research and Education, Chesapeake Biological Laboratory, Solomons, Md.